Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Nedd4L suppression in lung fibroblasts facilitates pathogenesis of lung fibrosis

doi: 10.1016/j.trsl.2022.10.002

Figure Lengend Snippet: A. Nedd4L mRNA levels in controls (n=108) compared to IPF group (n=160). Mann Whitney test, P<0.001. B. Expression of Nedd4L in arbitrary units as a function of diffusing capacity of carbon monoxide (DLCO)% predicated (IPF patients, n=145). C. Expression of Nedd4L in arbitrary units as a function of FVC% predicated. (IPF patients, n=157). D. IHC staining of Nedd4L in lung tissues from IT PBS and IT bleomycin (BLM)-challenged mice. Nedd4L positive intensities were analyzed by Image J. E.F., Immunoblotting analysis of lung tissues of BLM-challenged mice. Nedd4L intensities were analyzed by Image J (n=3−4). G. A negative correlation between expression of Nedd4L and FN in lung tissues from BLM-challenged mice. H. Nedd4L mRNA levels from lungs of IT PBS- or IT BLM (2 U/kg, 14 d)-challenged mice were examined by realtime PCR. I. Representative immunoblots of lung fibroblasts from normal control subjects and IPF patients. Nedd4L intensities were analyzed by Image J (n=4−6). J. Representative immunoblots in isolated lung fibroblast cells from IT PBS and IT BLM-challenged mice (n=3−4). K. Flow cytometry analysis of Nedd4L in isolated lung fibroblasts (cd326−/cd45−/cd31−/cd140a+) from IT PBS (cont)- or IT BLM-challenged mice. Gray: Cont; red: IT BLM-treated cells. Shown are representative flow cytometry histograms. n=3−4, P<0.01.

Article Snippet: Human lung fibroblast cell lines (Mrc5 and IMR90) were purchased from ATCC (Manassas, VA).

Techniques: MANN-WHITNEY, Expressing, Immunohistochemistry, Western Blot, Control, Isolation, Flow Cytometry

Journal: Translational research : the journal of laboratory and clinical medicine

Article Title: Nedd4L suppression in lung fibroblasts facilitates pathogenesis of lung fibrosis

doi: 10.1016/j.trsl.2022.10.002

Figure Lengend Snippet: A. Immunoblotting analysis of isolated lung fibroblast cells from IT lenti-Nedd4L-V5-transducted mice. B. IHC staining of mouse lung tissues from lenti-control and lenti-Nedd4L-V5-transduced mice with a V5 antibody. n=3. C. Immunoblotting analysis of lung tissue lysates. FN intensities were analyzed by Image J. (n=3). D. Ashcraft score of lung histology. n=6−8. E. Masson trichrome staining of lung tissues. n=4−5. F. Collagen content in the right lung was analyzed by Sircol collagen assay. n=6−8.

Article Snippet: Human lung fibroblast cell lines (Mrc5 and IMR90) were purchased from ATCC (Manassas, VA).

Techniques: Western Blot, Isolation, Immunohistochemistry, Control, Staining, Sircol Collagen Assay

Journal: Journal of Cellular and Molecular Medicine

Article Title: MCM4 acts as a biomarker for LUAD prognosis

doi: 10.1111/jcmm.17819

Figure Lengend Snippet: Elevated expression of MCM4 was observed in LUAD tissues and cells, which caused low overall survival rate. (A) MCM4 expression in TCGA datasets. MCM4 level in LUAD tumour samples was higher than that of adjacent normal tissues. (B) The overall survival rate of patients with high MCM4 expression was reduced compared with that with low MCM4 expression. (C) Cox univariate regression analysis of age and histological grade in TCGA cohort. (D) Western blotting assay showed MCM4 level was increased in tumour tissues in relative to those in normal tissues in LSL‐Kras G12D/+ mice model. (E) MCM4 was overexpressed in LUAD cells (H441, H460 and H552) compared with human embryonic lung fibroblast cells (MRC5 and WI38). * p < 0.05. N, adjacent normal tissues; T, tumour tissues. (F) Quantitative results of MCM4 expression.

Article Snippet: Four human LUAD cell lines (H441, H460, H522, A549) and two human embryonic lung fibroblast cell lines (WI38 and MRC5) were obtained from the American Type Culture Collection (ATCC).

Techniques: Expressing, Western Blot

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Strains and plasmids used in this study .

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Growth assays in different YNB media. (A) Deletion of CgASG1 has no effect on the utilization of non-fermentative carbon sources in C. glabrata . (B) CgAsg1p plays a role under acid-stress conditions. (C) CgHAL9 serves an important role in cell growth under hypersaline conditions in C. glabrata . (D) CgHal9p plays a role under acid-stress conditions. Logarithmic-phase cells of each C. glabrata strain were adjusted to 2 × 10 7 cells/mL, and then 4 μL of serial tenfold dilutions were spotted onto the corresponding YNB media, as indicated. Pictures were taken after 4 days of growth at 30°C.

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Intracellular pH of wild-type, mutant and complemented strains of C. glabrata as below ^a .

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Transcription factors Asg1p and Hal9p regulate pH homeostasis in Candida glabrata

doi: 10.3389/fmicb.2015.00843

Figure Lengend Snippet: Quantitative reverse-transcribed PCR (qRT-PCR) validation of transcriptional profiles in an acidic environment . Logarithmic-phase C. glabrata cells were incubated in YNB or YNB-pH 2.0 media for 2 h. qRT-PCR analyses of the indicated genes was performed as described in the Materials and Methods Section. The means and standard deviations for three independent experiments are shown. C. glabrata strains: wild-type, Cgasg1 Δ and Cghal9 Δ strains. ( * P < 0.05 compared to the corresponding wild-type control, as determined by t -test).

Article Snippet: C. glabrata ATCC 55/ GFP , C. glabrata ATCC 55 (pY13- GFP ) , This study.

Techniques: Reverse Transcription, Quantitative RT-PCR, Biomarker Discovery, Incubation, Control

Journal: Nature Communications

Article Title: Multifunctional hydrogel nano-probes for atomic force microscopy

doi: 10.1038/ncomms11566

Figure Lengend Snippet: ( a ) Contact mode height images, with fixed 3 nN contact forces, for human fibroblast cells (MRC-5) in PBS imaged by a commercial silicon probe (first row) and a fabricated hydrogel probe (second row) at various scan rates. All scale bars are 5 μm. ( b ) Height profiles along the A–A′ and B–B′ lines in a . ( c ) Contact mode height images, with fixed 300 nN contact force, for MRC-5 cells in PBS sequentially imaged by using hydrogel (first), silicon (second) and hydrogel (third) probes at a scan rate of 1 Hz. All scale bars are 5 μm. ( d ) Height profiles along the A–A′ line in c .

Article Snippet: Human breast cancer cells (MCF-7, Sigma), human fibroblast cells (MRC-5, Japanese Collection of Research Bioresources Cell Bank; JCRB Cell Bank) and HeLa cells (ATCC CCL-2, ATCC) were used in experiments.

Techniques: